RNA Crosslinking and Click Chemistry with Platinum Compounds

Platinum-RNA Modifications Following Drug Treatment in S. cerevisiae Identified by Click Chemistry and Enzymatic Mapping

With the importance of RNA-based regulatory pathways, the potential for targeting noncoding and coding RNAs by small molecule therapeutics is of great interest. Platinum(II) complexes including cisplatin (cis-diamminedichloroplatinum(II)) are widely prescribed anticancer compounds that form stable adducts on nucleic acids. In tumors, DNA damage from Pt(II) initiates apoptotic signaling, but thi...

Click chemistry with DNAw

Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry.

Two new dibenzocyclooctyne-thymidine monomers were incorporated into oligonucleotides and crosslinked to azide-labelled complementary strands across the DNA grooves. Equivalent reactions were successful using a (bicyclo[6.1.0]nonyne) alkyne. Oligonucleotides containing internal cyclooctyne and amino groups were simultaneously reacted with azides and NHS esters of different fluorescent dyes to p...

Visualizing coronavirus RNA synthesis in time by using click chemistry.

Coronaviruses induce in infected cells the formation of replicative structures, consisting of double-membrane vesicles (DMVs) and convoluted membranes, where viral RNA synthesis supposedly takes place and to which the nonstructural proteins (nsp's) localize. Double-stranded RNA (dsRNA), the presumed intermediate in RNA synthesis, is localized to the DMV interior. However, as pores connecting th...

Copper-free click chemistry for the in situ crosslinking of photodegradable star polymersw

Covalently crosslinked polymeric materials which swell, but do not dissolve, in a given solvent (polymer gels) have found utility in a number of applications including tissue engineering, drug delivery, chemical sensing, microfluidics, cosmetics, and microelectronics. End-linked polymer gels (model networks or MNs) are especially promising for drug delivery applications due to their easily cont...